Atomic Force Microscope - BioScienceAmpliar
JPK NanoWizard 3 - INL operates an atomic force microscope (AFM) optimized for bioscience, i.e. designed for optimal operation in liquid media. It provides a vapour barrier, encapsulated piezos and a variety of dedicated liquid cells for applications ranging from single molecules to living cells. The JPK Nanowizard 3 is integrated with an inverted optical microscope (Ti-S, Nikon) via suitable condenser optics and a fully motorized sample translation stage. The JPK HyperDrive mode-of-operation provides the capability for imaging soft samples in liquid at sub-nm resolution with minimized tip-sample interactions and the JPK Quantitative Imaging (QI) mode performs AFM imaging without exerting lateral force, ideal for soft, sticky, or loosely attached samples. At the end of September 2013, the JPK NanoWizard 3 has been integrated by INL with a new type of fluorescence sectioning module (DSD, Andor Technology), providing depth-resolved fluorescence imaging with a metal-halide broadband white light source that distinguishes this optical sectioning apparatus from a laser scanning confocal microscope. The JPK DirectOverlay software module provides super-pixel registration of fluorescence and AFM images.