Confocal Laser Scanning MicroscopeAmpliar
Manufaturer: Zeiss; Model: LSM780. INL operates a laser scanning confocal microscope optimized for the study of cellular fluorescence. Fluorescence excitation is achieved through a laser rack with an Argon laser and several solid state lasers, providing a total of six excitation wavelengths covering the visible spectrum (405, 488, 514, 532, 561 and 633nm). Fluorescence is detected by the Zeiss 34 channel Quasar detector, consisting of a regular multi-alkali photomultiplier tube (PMT), a cooled PMT and a 32 channel Gallium-Arsenide-Phosphide (GaAsP) detector, providing state-of-the-art detection in detection sensitivity for confocally detected fluorescence. Transmitted light image formation can be performed in confocal and wide-field mode through a transmitted light PMT detector and differential interference contrast mechanism respectively. The study of live cells over long periods of time is made possible through an integrated climate chamber providing a 37oC and 5% CO2 atmosphere. A user-friendly software package provides highly automated data acquisition and image processing functionality.