Nanomedicine Nonlinear Effects and Bioimaging

Nonlinear effects, such as second harmonic generation or multi-photon excitation are known to be advantageous for noninvasive biological imaging. While second harmonic generation is sensitive to a small but relevant number of biological components, for example neurons, axons, collagen and starch which renders it an excellent technique for the neurosciences, medicine and food quality control, multi-photon excitation on the other hand has optical advantages compared to the linear counterpart as it allows the use of infrared light which is able to penetrates deeper into tissue compared to light in the visible range, while translating the signal into the well detectable visible range.

Using advanced tools to generate and control ultrafast femtosecond pulse characteristics we envision a new generation of nonlinear microscopy characterized by providing enhanced imaging contrast. 

Strategies to use nonlinear nanocrystals as biomarkers shall be developed and tested.


Illustration of the different effects when combining controlled femtosecond pulses with a scanning microscope setup.

Collaboration partner: Prof. Dr. Helder Crespo from University of Porto