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The Nanophotonics Department at INL provides expertise in the analysis of light-matter interactions at various scales: Reaching from the detection of individual fluorescent molecules or nanoparticles to the high-resolution label-free and fluorescence-based imaging of fixed and life cells, e.g. in 2D cell models or tissues.
Furthermore we are experienced to design optically active nanomaterials and characterize e.g. plasmonic interaction effects or surface enhanced Raman effects (SERS) and study optical properties of metamaterials.

Our Nanophotonics Flagship Facility provides a comprehensive set of high-end commercial solutions for imaging and optical spectroscopy suited for the characterization of biological samples and the characterization of new materials.
Furthermore, our researchers are developing new generation imaging and spectroscopy solutions that:

• Are characterized by multimodality, for faster and more comprehensive analysis of your samples and allowing correlative imaging analysis
• Involve the use of INL-developed innovative contrast agents
• Allow optical super-resolution – surpassing the typical few hundreds of nanometer resolution in diffraction-limited classical microscopy and approaching the nanometer scale.

Our interdisciplinary team of physicists, biologists, biomedical engineers and chemists is continuously developing new Nanophotonics techniques that shall help advance our understanding of complex biological and non-biological matter.

Examples of research fields to which the researchers of the INL Nanophotonics department contributes are:

• Toxicology
• Drug delivery systems
• Plasmonic interaction effects and metamaterials
• Nanomedicine
• Biophysics
• Optical and plasmonic sensing technologies
• Quantum Sensing Technologies
• Development of super resolution imaging techniques
• Development of combined microscopies (topography/fluorescence)
• Development of optical active structures for technological applications (LEDs, solar cells)

Techniques available

• Confocal Light Scanning Microscope (Life cell- option) (Zeiss LSM780)

• Wide Field Microscope (Life Cell – option) (Nikon Ti)

• Total Internal Reflection Microscopy (TIRF) ((Nikon Ti upgrade)

• Super Resolution Microscope – dSTORM (Nikon Ti upgrade)

• Confocal Raman Microscope (WiTec)

• Fluorescence Brightfield microscope (Nikon)

• Flow cytometer/Cell sorter (BioRad)

• Femtosecond Laser-Based Microfabrication (Spectra Physics Tsunami, Newport uFAB)

• Combined mechanical and optical characterization: AFM/Differential Spinning Disk

• Fluorescence lifetime imaging microscopy (FLIM) setup with single molecule sensitivity, suited for life cell observation, fluorescence anisotropy detection and suited for nanomedicine research such as the investigation of photothermal or hyperthermia therapy effects on cells

• Multiphoton/Second Harmonic Generation microscopy

• Spectral imaging ellipsometry

• Fluorescence lifetime spectroscopy (TCSPC based)(ISS ChronosBH)
• Fluorescence anisotropy (ISS Chronos BH)
• Fluorescence lifetime and energy transfer detection (Streak camera based) (Hamamatsu)
• Circular Dichroism Spectroscopy (Jasco)
• FTIR spectroscopy (Bruker)
• Dynamic Light Scattering (Horiba)
• Fluorescence (Cross) Correlation Spectroscopy (F(C)CS) (as upgrade of the Zeiss LSM780)
• Ellipsometry coupled with Quartz Crystal Balamce

For more information please contact:

Photo - Camilo Guzman web

Dr. Camilo Guzmán

Facility Manager Nanophotonics and Bioimaging

tel. +351 253 140 112 (2502)