Instruct Hands-on Workshop

The Instruct Hands-on Workshop, held from October 7 to 10, 2024, is set to provide in-depth training on sample preparation for single particle analysis (SPA) by cryo-electron microscopy (cryo-EM). The workshop, hosted by a team of experts from ITQB NOVA, INL, and CNB–CSIC, aims to equip participants with best practices and the latest techniques in cryo-EM imaging.

Participants will engage in a comprehensive course featuring a blend of lectures and hands-on tutorials. The lectures will delve into the principles of SPA, covering theoretical and practical aspects of preparing biological samples for negative staining and quality assessment. Practical sessions will offer hands-on experience with vitrification using a plunge-freezing device and optimising grids for sample screening with a cryo-transmission electron microscope (TEM).

Attendees are encouraged to bring their samples, providing a unique opportunity to apply newly acquired skills to their specific research needs. The workshop will highlight recent advancements in protein production and stabilisation, as well as the latest technological innovations in grid preparation and sample manipulation. This integrative approach will be crucial for addressing challenging scientific projects.

The workshop aims to provide participants with a holistic understanding of the integrated use of biophysics, biochemistry, and cryo-EM for protein structure determination and analysis. By the end of the course, attendees will have a firm grasp of the individual aspects of sample preparation for SPA by cryo-EM, ready to apply these techniques to their research endeavours.

This workshop is a unique opportunity for researchers to enhance their skills and stay at the forefront of structural biology. Don’t miss the chance to join this valuable training event.

Organising Committee:
José A. Brito (ITQB NOVA)
Margarida Archer (ITQB NOVA)
Oliver Schraidt (INL)
Paulo Ferreira (INL)
Jose Maria Carazo (CNB–CSIC)