The main interest of Ernesto Alfaro-Moreno lies in evaluating local and systemic effects induced by PM10, PM2.5, and nanoparticles. He has been focused on characterizing the toxicity of particles while evaluating the effects on epithelial, endothelial, and monocytic cells. He has been very active in the characterization of Cytotoxic, genotoxic, and proinflammatory effects. He also has been involved in the development and improvement of new In vitro models. His current interest in the Nanosafety area include the development of alveolo-vascular communication models, models for skin-on-a-chip, multi-sensing, C. elegans, and in silico assessments of nanoparticles and complex mixtures.