ON4SupremeSens

The modifications of the fluorescence lifetime by the presence of fluorescence quenchers can be used to increase the axial resolution of fluorescence lifetime imaging microscopy (FLIM). The axial spacial range of the quencher material depends on its refractive index (RI), so matherials with different RI can be explored in order to improve and modify the imaging axial range and axial resolution of FLIM. The main goal of the project is to optimize the FLIM technique for different resolutions and distance ranges to study different biological processes. Different materials will be used to manipulate the spatial range and resolution of the tested materials. Concerning the applications of the optimized technique, one goal is to visualize the hybridization of DNA nanostructures when in contact with olive oil DNA strands. The other biological process that will be addressed is the visualization of epithelial cell?s tight junctions (TJ) in order to monitor their function and permeability.