WOMENINSCIENCE-13

Food Quality and Safety

RESEARCH

HIGHLIGHTS

Paper published in Food Microbiology

Rapid and sensitive detection of viable Listeria monocytogenes in food products by a filtration-based protocol and qPCR. Alejandro Garrido-Maestu,Sarah Azinheiro, Joana Carvalho, Marta Prado. Volume 73, August 2018, Pages 254–263.

Paper published in Microchemical Journal

Novel approach for accurate minute DNA quantification on microvolumetric solutions

JoanaCarvalho,RenatoNegrinho,SarahAzinheiro, AlejandroGarrido-Maestu,JorgeBarros-Velázquez.MartaPrado.Volume 138,May 2018,Pages 540-549.

Published a Research Article in Front. Sustain. Food Syst.

Evaluation of Different Genetic Targets for Salmonella enterica Serovar Enteriditis and Typhimurium, Using Loop-Mediated Isothermal AMPlification for Detection in Food Samples

SarahAzinheiro,JoanaCarvalho,MartaPrado and AlejandroGarrido-Maestu. 21 February 2018.

Paper published in Analytica chimica Acta.

Highly efficient DNA extraction and purification from olive oil on a washable and reusable miniaturized device.

JoanaCarvalho,GemaPuertas,JoãoGaspar,
SarahAzinheiro,LorenaDiéguez,
AlejandroGarrido Maestu, ManuelVázquez,
JorgeBarros-Velázquez,SusanaCardoso,MartaPrado.

Volume 1020, 22 August 2018, Pages 30-40

Scientific Opinion !

The EFSA (European Food Safety Authority) has recently published a scientific opinion on an “Updated quantitative risk assessment (QRA) of the BSE risk posed by processed animal protein (PAP)” & We are happy to mention that Our Food Quality & Safety research Group leader Dr.Marta Prado has participated in this activity Find the links at The Wiley Online Library (WOL) & The EFSA website

Picture4_Marta (1)

Published a Research Article in Food Control

Specific detection of viable Salmonella Enteritidis by phage amplification combined with qPCR (PAA-qPCR) in spiked chicken meat samples

Author: Alejandro Garrido-Maestu, PabloFuciños, SarahAzinheiro, CarlaCarvalho, JoanaCarvalho, MartaPrado

Food Control 99 , pp. 79-83, 2019.

Book Chapter in collaboration with David Tomás Fornés, Lead Scientist in the Microbial and Molecular Analytics group at Nestlé Research Center (Lausanne, Switzerland). !!!!!! Foodborne Bacterial Pathogens

The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens In: Bridier A. (eds) Foodborne Bacterial Pathogens. Methods in Molecular Biology, vol 1918. Humana Press, New York, NY

Reliable Nano & Micro-solutions for Food Safety & Quality Analysis

The main objective of the Food & Quality Research Group is the development of analytical approaches based on the combination of molecular biology (mainly DNA based methodology) and nano and microfabrication technology in order to provide the food industry and control laboratories with reliable analytical tools.

Following this objective, the methodology is based on working on very specific analytical needs and on using a modular approach for each of the steps of the analytical process. This approach, help us to evaluate and to choose the best method in each case, to have a sound integrated final product, and at the same time a wide-range of intermediate products that can be used by themselves to solve specific analytical challenges.

Figure 1 summarizes the overall approach and research lines. Our topics of interest involve the detection of foodborne pathogens, the detection of allergenic ingredients in food products and food authenticity.

RESEARCH LINES

  • Sample preparation:

Sample preparation is the series of steps required to transform a sample to a form suitable for analysis, the reliability of the conclusions drawn from food analysis greatly depends upon on this step. We work on: (i) the development of pre-treatment steps in order to overcome some of the limitations associated with food analysis and (ii) on the development of tailored, miniaturized, automatized and faster sample preparation techniques. Microscale solid phase extraction (µSPE) is used for on-chip DNA extraction and purification, being possible to put in contact a higher volume of initial binding material with the solid phase and recover the DNA in a lower volume during the elution phase. This feature allows to concentrate the DNA when minute amounts are present in the sample (e.g. olive oil, wine), for complex matrixes such as processed foodstuff and for environmental samples (e.g. water samples).

  • Alternative DNA amplification methods:

Food & Quality Safety Research Group is working on new amplification techniques, in their combination with NPs and on the evaluation of DNA based analytical methods for food analysis. We work on isothermal amplification techniques, such as Loop-Mediated Isothermal Amplification (LAMP), and Recombinase Polymerase Amplification (RPA), specially interesting for miniaturization purposes. Other alternative techniques currently being used include Ligation Chain Reaction (LCR) which allows to distinguish very closely related organisms and high similar DNA sequences.

  • Nanoparticle-assisted DNA analysis:

The use of nanomaterials for DNA analysis has the potential of providing increased sensitivity, multiplexing capabilities, and reduced costs. Exploiting the features of nanoparticles (NPs) is considered to be a good alternative to foster the potential of diagnostics and analytical method development. NPs, such as gold NPs (AuNPs) and gold nanorods (AuNRs) are being used for DNA detection taking advantage of their optical properties.

INL_GroupPhoto-Food Quality and safety

Food Quality and Safety research team (picture from April 2019)

FUNDED PROJECTS

DEVELOPMENT OF A SYSTEM OF EARLY DETECTION OF THE ZEBRA MUSSEL THROUGH ANALYSIS OF ENVIRONMENTAL DNA

Cooperation Agreement with Confederación hidrográfica del Guadalquivir (CHG) Spain.

The objective of this cooperation agreement is the development of a micro Total Analysis Systems (µTAS) and optimized protocol for the detection of zebra mussel, an invasive species affecting numerous river basins worldwide, through environmental DNA (eDNA).

NANOEATERS: Valorization and transfer of NANOtechnologies to EArly adopTERS of the Euroregion Galicia-Norte Portugal-Use Case 2: Olive Oil Characterization

The FQ&S research group participates on the NANOEATERS project, on the Use Case 2: Olive Oil characterization on collaboration with the University of Vigo (Spain).
The main objective of use case 2 is the characterization of Extra virgin olive oil produced in Galicia, and the development of analytical approaches that would allow the differentiation of such olive oil from the one produced elsewhere.

N2020- NBFS-FOODSAFE

Innovative sample preparation and detection methods for foodborne pathogens, allergenic ingredients and food authenticity assessment are being developed. In parallel, new smart packaging and delivery systems are being designed and validated.

PORTGRAPHE

Control of Port and Douro Wines authenticity using graphene DNA sensors project

Time Frame: June 2018 to June 2021

The main objective of the project is the development, test and in-house validation of a miniaturized DNA sensing device for varietal discrimination of grapes, wines, musts, and grape juice in order to ensure the authenticity of wine from Port and Douro DOP. With this objective the participating teams will combine their expertise for the development of a miniaturized analytical device composed of 3 modules namely: a DNA extraction and purification module, an isothermal DNA amplification module, and a DNA Biosensors based on field-effect transistors (FETs) made using single layer graphene (SLG) for varietal discrimination.

NanoBioSensor

Development of nanosensors to evaluate the microbiological quality of fruit-based products

Time Frame: July 2018 to June 2021

A micro-total analysis system will be developed by the participating team to enable faster and better control of selected fruit-based products, reducing the analysis time from 7 days to few hours with greater sensitivity than conventional methods. This device will be designed and manufactured combining the latest developments in molecular biology, microfluidics and electronics.

SF4SF Smart Factoy for Safe Foods

 

Project funded by CDTI, Ministry of Industry of Spain.

The project aims the integration of emerging technologies for the detection, removal and food hazards management in food processing plants. The role of the group is the development of analytical methods based on DNA detection from gluten producing cereals and Listeria monocytogenes in fish products.

Innovative Consumer OrieNted Safe Solutions (ICONSS. Project_ 20431) Funding from EITFood BP2020

 

The ICONSS project objective is the development of an integrated solution targeting consumers and food operators. This integrated solution is made up of two modules: the first one, an app that will look at nutritional aspects for vulnerable population groups and create a food box from retailers ready to pick up or for home delivery, to minimize exposure in indoor environments particularly for vulnerable people. The second module, also connected with an app, will be a miniaturized device for fast, reliable and easy to use testing of workers and surfaces to ensure the safety in food-related environments, with special emphasis on retailers. This miniaturized kit and device will include sampling, a simplified RNA extraction and isothermal amplification and detection, ready to be used on decentralized settings with minimum interaction from users, and giving a simple and easy to read answer.

Webpage https://iconss.eu/

CRISPR technology against the COVID-19 pandemic. The DETECTR of SARS-CoV-2 (project n. 480) funding from FCT (Portugal)

 

COVID-19 pandemic is still on-going, affecting 4 million people. The viral detection relies on RT-qPCR that is sensitive and specific, but takes long time to results, needs special equipment and trained personnel. Thus, there is a need for methods equally sensitive and specific. DETECTR technology, combining RPA and CRISPR-Cas12a can fulfill these requirements. We will use it to develop a multiplex lateral flow method for naked-eye detection of the virus in only 30 min.

LAMP‐Light in the diagnosis of COVID‐19 (project n. 165) funding from FCT (Portugal)

 

SARS‐CoV‐2 virus is rapidly spreading. The standard to detect it is RT‐qPCR, but the method is lengthy and needs expensive equipment. In this project we will develop a SARS‐CoV‐2 detection methodology based on RT‐LAMP. The technique has a fast turnaround time, does not need specialized equipment, and detection can be performed by naked‐eye. Thus the proposed method has great potential to enhance diagnostics throughput, reduce time of analysis, and improve implementation of appropriate measures.

PUBLICATIONS

  • 2021

    Azinheiro, S., Ghimire, D., Carvalho, J., Prado, M., & Garrido-Maestu, A. (2022)

    Next-day detection of viable Listeria monocytogenes by multiplex reverse transcriptase real-time PCR Journal Article

    Food Control, 133 (PA) , 2021.

    Abstract | Links | BibTeX

    Azinheiro, S., Roumani, F., Carvalho, J., Prado, M., & Garrido-Maestu, A.

    Suitability of the MinION long read sequencer for semi-targeted detection of foodborne pathogens Journal Article

    Analytica Chimica Acta, 1184 , 2021.

    Abstract | Links | BibTeX

    Guerreiro, J. R. L., Ipatov, A., Carvalho, J., Toldrà, A., & Prado, M.

    Amplified plasmonic and microfluidic setup for DNA monitoring Journal Article

    Microchimica Acta, (2021), (326), 2021.

    Abstract | Links | BibTeX

    Azinheiro, S., Roumani, F., Rodríguez-Lorenzo, L., Carvalho, J., Prado, M., & Garrido-Maestu, A.

    Combination of Recombinase Polymerase Amplification with SYBR Green I for naked-eye, same-day detection of Escherichia coli O157:H7 in ground meat Journal Article

    Food Control (2022), 132 , 2021.

    Abstract | Links | BibTeX

    Elumalai, M., Ipatov, A., Carvalho, J., Guerreiro, J., Prado, M.

    Dual colorimetric strategy for specific DNA detection by nicking endonuclease-assisted gold nanoparticle signal amplification Journal Article

    Anal Bioanal Chem, 2021.

    Abstract | Links | BibTeX

    Carvalho, J.; Yadav, S.; Garrido-Maestu, A.G.; Azinheiro, S.; Trujillo, I.; Barros-Velázquez, J.; Prado, M.

    Evaluation of simple sequence repeats (SSR) and single nucleotide polymorphism (SNP)-based methods in olive varieties from the Northwest of Spain and potential for miniaturization Journal Article

    Food Chemistry: Molecular Sciences, 3 , 2021.

    Abstract | Links | BibTeX

    Maryam Naseri ,Arnab Halder ,Mohsen Mohammadniaei ,Marta Prado ,Jon Ashley ,Yi Sun

    A multivalent aptamer-based electrochemical biosensor for biomarker detection in urinary tract infection Journal Article

    Electrochimica Acta (2021), 389 , 2021.

    Abstract | Links | BibTeX

    Roumani, F.; Azinheiro, S.; Sousa, H.; Sousa, A.; Timóteo, M.; Varandas, T.; Fonseca-silva, D.; Baldaque, I.; Carvalho, J.; Prado, M.; Garrido-Maestu

    Optimization and Clinical Evaluation of a Multi-Target Loop-Mediated Isothermal Amplification Assay for the Detection of SARS-CoV-2 in Nasopharyngeal Samples Journal Article

    Viruses 2021, 2021.

    Abstract | Links | BibTeX

    Carvalho J and Maestu AG and Azinheiro S and Fuciños P and Velázquez J B and Miguel RJ De and Gros V and Prado M

    Faster monitoring of the invasive alien species ( IAS ) Dreissena polymorpha in river basins through isothermal amplification Journal Article

    Scientific Reports, 11 (10175), 2021.

    Abstract | Links | BibTeX

    Ipatov, A and Garrido- Maestu, A and Guerreiro, J R and Purwidyantri, A and Azinheiro, S and Carvalho, J and Romani, F and Elumalai, M and Prado, M

    Application of Omics-based Miniaturized Systems in Food Quality and Safety Book Chapter

    2021, ISBN: 978-1-78801-884-5.

    Abstract | Links | BibTeX

    Carvalho J and Diéguez L and Ipatov A and Guerreiro J R and Garrido-Maestu A and Azinheiro S and Prado M

    Single-use microfluidic device for purification and concentration of environmental DNA from river water Journal Article

    Talanta, 226 , 2021.

    Abstract | Links | BibTeX

    Purwidyantri A and Domingues T and Borme J and Guerreiro J R and Ipatov A and Abreu C M and Martins M and Alpuim P and Prado M

    Influence of the Electrolyte Salt Concentration on DNA Detection with Graphene Transistors Journal Article

    Biosensors, 11 , pp. 24, 2021.

    Abstract | Links | BibTeX

    Roumani F and Azinheiro S and Carvalho J and Prado M and Garrido-maestu A

    Loop-mediated isothermal amplification combined with immunomagnetic separation and propidium monoazide for the specific detection of viable Listeria monocytogenes in milk products , with an internal amplification control Journal Article

    Food Control, 125 , 2021.

    Abstract | Links | BibTeX

    Jon Ashley, Anna-Lisa Schaap-Johansen, Mohsen Mohammadniaei, Maryam Naseri, Paolo Marcatili, Marta Prado, Yi Sun

    Terminal deoxynucleotidyl transferase-mediated formation of protein binding polynucleotides Journal Article

    Nucleic Acids Research, 2021.

    Abstract | Links | BibTeX

  • 2020

    A Garrido-Maestu and S Azinheiro and F Roumani and J Carvalho and M Prado

    Application of Short Pre-enrichment, and Double Chemistry Real-Time PCR, Combining Fluorescent Probes and an Intercalating Dye, for Same-Day Detection and Confirmation of Salmonella spp. and Escherichia coli O157 in Ground Beef and Chicken Samples Journal Article

    Front. Microbiol, 11 , pp. 1-8, 2020.

    Abstract | Links | BibTeX

    L Wu and A Teixeira and A Garrido-Maestu and L Muinelo-Romay and L Lima and LL Santos and M Prado and L Diéguez

    Profiling DNA mutation patterns by SERS fingerprinting for supervised cancer classification Journal Article

    Biosens. Bioelectron, 165 , 2020.

    Abstract | Links | BibTeX

    S Azinheiro and J Carvalho and M Prado and A Garrido-Maestu

    Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces Journal Article

    Foods, 9 , 2020.

    Abstract | Links | BibTeX

    S Azinheiro and J Carvalho and M Prado

    Multiplex Detection of Salmonella spp., E. coli O157 and L. monocytogenes by qPCR Melt Curve Analysis in Spiked Infant Formula Journal Article

    Microorganisms, 8 , 2020.

    Abstract | Links | BibTeX

    AJN Oliveira and J de Wild and K Oliveira and BA Valença and JP Teixeira and JRL Guerreiro and S Abalde-Cela and TS Lopes and RM Ribeiro and JM V Cunha and MA Curado and M Monteiro and A Violas and AG Silva and M Prado and PA Fernandes and B Vermang and PMP Salomé

    Encapsulation of Nanostructures in a Dielectric Matrix Providing Optical Enhancement in Ultrathin Solar Cells Journal Article

    Sol. RRL., 4 , 2020.

    Abstract | Links | BibTeX

    S Azinheiro and K Kant and MA Shahbazi and A Garrido-Maestu and M Prado and L Diéguez

    A smart microfluidic platform for rapid multiplexed detection of foodborne pathogens Journal Article

    Food Control, 114 , 2020.

    Abstract | Links | BibTeX

    Garrido-Maestu A and Azinheiro S and Carvalho J and Espiña B and Prado M

    Evaluation and implementation of commercial antibodies for improved nanoparticle-based immunomagnetic separation and real-time PCR for faster detection of Listeria monocytogenes Journal Article

    Food Sci. Technol., (57), pp. 4143–4151, 2020.

    Abstract | Links | BibTeX

    A Teixeira and JL Paris and F Roumani and L Diéguez and M Prado and B Espiña and S Abalde-Cela and A Garrido-Maestu and L Rodriguez-Lorenzo

    Multifuntional gold nanoparticles for the SERS detection of pathogens combined with a LAMP-in-microdroplets approach Journal Article

    Materials (Basel), 13 , 2020.

    Abstract | Links | BibTeX

    A Garrido-Maestu and S Azinheiro and J Carvalho and P Fuciños and M Prado

    Optimized sample treatment, combined with real-time PCR, for same-day detection of E. coli O157 in ground beef and leafy greens Journal Article

    Food Control, 108 , 2020.

    Abstract | Links | BibTeX

  • 2019

    L Rodriguez-Lorenzo and A Garrido-Maestu and AK Bhunia and B Espiña and M Prado and L Diéguez and S Abalde-Cela

    Gold Nanostars for the Detection of Foodborne Pathogens via Surface-Enhanced Raman Scattering Combined with Microfluidics Journal Article

    ACS Appl. Nano Mater., 2 (10), pp. 6081–6086, 2019.

    Abstract | Links | BibTeX

    Carla Carvalho Joana Carvalho Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Combination of Immunomagnetic separation and real-time Recombinase Polymerase Amplification (IMS-qRPA) for specific detection of Listeria monocytogenes in smoked salmon samples Journal Article

    Food Science, 84 (7), pp. 1881-1887, 2019.

    Abstract | Links | BibTeX

    Carla Carvalho Joana Carvalho Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Combination of Immunomagnetic separation and real-time Recombinase Polymerase Amplification (IMS-qRPA) for specific detection of Listeria monocytogenes in smoked salmon samples Journal Article

    Food Science, 84 (7), pp. 1881-1887, 2019.

    Abstract | Links | BibTeX

    SarahAzinheiro CarlaCarvalho JoanaCarvalho MartaPrado Alejandro Garrido-Maestu PabloFuciños

    Specific detection of viable Salmonella Enteritidis by phage amplification combined with qPCR (PAA-qPCR) in spiked chicken meat samples Journal Article

    Food Control, 99 , pp. 79-83, 2019.

    Abstract | Links | BibTeX

    SarahAzinheiro CarlaCarvalho JoanaCarvalho MartaPrado Alejandro Garrido-Maestu PabloFuciños

    Specific detection of viable Salmonella Enteritidis by phage amplification combined with qPCR (PAA-qPCR) in spiked chicken meat samples Journal Article

    Food Control, 99 , pp. 79-83, 2019.

    Abstract | Links | BibTeX

    Joana Guerreiro Sandra Carvalho Sara Abalde-Cela Marta Prado Lorena Diéguez R L Lei Wu Alejandro Garrido-Maestu

    Amplification-free SERS analysis of DNA mutation in cancer cells with single-base sensitivity Journal Article

    Nanoscale, 11 (16), pp. 7781-7789, 2019.

    Abstract | Links | BibTeX

    Joana Guerreiro Sandra Carvalho Sara Abalde-Cela Marta Prado Lorena Diéguez R L Lei Wu Alejandro Garrido-Maestu

    Amplification-free SERS analysis of DNA mutation in cancer cells with single-base sensitivity Journal Article

    Nanoscale, 11 (16), pp. 7781-7789, 2019.

    Abstract | Links | BibTeX

  • 2018

    Marta Prado Rodríguez Alejandro Garrido-Maestu David Tomás Fornés

    The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens Book Chapter

    Methods in Molecular Biology, 1918 , pp. 35-45, Humana Press, New York, NY, 2018.

    Abstract | Links | BibTeX

    Marta Prado Rodríguez Alejandro Garrido-Maestu David Tomás Fornés

    The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens Book Chapter

    Methods in Molecular Biology, 1918 , pp. 35-45, Humana Press, New York, NY, 2018.

    Abstract | Links | BibTeX

    SarahAzinheiro AlejandroGarrido-Maestu JorgeBarros-Velázquez MartaPrado JoanaCarvalho RenatoNegrinho

    Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy Journal Article

    Elsevier, 21 (21), pp. 424-431, 2018.

    Abstract | Links | BibTeX

    SarahAzinheiro AlejandroGarrido-Maestu JorgeBarros-Velázquez MartaPrado JoanaCarvalho RenatoNegrinho

    Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy Journal Article

    Elsevier, 21 (21), pp. 424-431, 2018.

    Abstract | Links | BibTeX

    JoãoGaspar SarahAzinheiro LorenaDiéguez AlejandroGarrido-Maestu ManuelVázquez JorgeBarros-Velázquez SusanaCardoso MartaPradoa JoanaCarvalho GemaPuertas

    Highly efficient DNA extraction and purification from olive oil on a washable and reusable miniaturized device Journal Article

    Analytica Chimica Acta, 1020 , pp. 30-40, 2018.

    Abstract | Links | BibTeX

    JoãoGaspar SarahAzinheiro LorenaDiéguez AlejandroGarrido-Maestu ManuelVázquez JorgeBarros-Velázquez SusanaCardoso MartaPradoa JoanaCarvalho GemaPuertas

    Highly efficient DNA extraction and purification from olive oil on a washable and reusable miniaturized device Journal Article

    Analytica Chimica Acta, 1020 , pp. 30-40, 2018.

    Abstract | Links | BibTeX

    Sarah Azinheiro Pablo Fuciños Joana Carvalho Marta Prado Alejandro Garrido-Maestu

    Highly sensitive detection of gluten-containing cereals in food samples by real-time Loop-mediated isothermal AMPlification (qLAMP) and real-time polymerase chain reaction (qPCR) Journal Article

    Food Chemistry, Volume 246 , pp. Pages 156–163, 2018.

    Abstract | Links | BibTeX

    Sarah Azinheiro Pablo Fuciños Joana Carvalho Marta Prado Alejandro Garrido-Maestu

    Highly sensitive detection of gluten-containing cereals in food samples by real-time Loop-mediated isothermal AMPlification (qLAMP) and real-time polymerase chain reaction (qPCR) Journal Article

    Food Chemistry, Volume 246 , pp. Pages 156–163, 2018.

    Abstract | Links | BibTeX

    Joana Carvalho Pablo Fuciños Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Development and evaluation of loop-mediated isothermal amplification, and Recombinase Polymerase Amplification methodologies, for the detection of Listeria monocytogenes in ready-to-eat food samples Journal Article

    Food Control, Volume 86 , pp. Pages 27–34, 2018.

    Abstract | Links | BibTeX

    Joana Carvalho Pablo Fuciños Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Development and evaluation of loop-mediated isothermal amplification, and Recombinase Polymerase Amplification methodologies, for the detection of Listeria monocytogenes in ready-to-eat food samples Journal Article

    Food Control, Volume 86 , pp. Pages 27–34, 2018.

    Abstract | Links | BibTeX

    Joana Carvalho Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Rapid and sensitive detection of viable Listeria monocytogenes in food products by a filtration-based protocol and qPCR Journal Article

    Food Microbiology, 73 , pp. 254-263, 2018.

    Abstract | Links | BibTeX

    Joana Carvalho Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Rapid and sensitive detection of viable Listeria monocytogenes in food products by a filtration-based protocol and qPCR Journal Article

    Food Microbiology, 73 , pp. 254-263, 2018.

    Abstract | Links | BibTeX

    Marta Prado Sarah Azinheiro Joana Carvalho and Alejandro Garrido-Maestu.

    Evaluation of different genetic targets for Salmonella enterica serovar Enteriditis and Typhimurium, using Loop-mediated isothermal AMPlification for detection in food samples Journal Article

    Frontiers in sustainable food systems, 2018.

    Abstract | Links | BibTeX

    Marta Prado Sarah Azinheiro Joana Carvalho and Alejandro Garrido-Maestu.

    Evaluation of different genetic targets for Salmonella enterica serovar Enteriditis and Typhimurium, using Loop-mediated isothermal AMPlification for detection in food samples Journal Article

    Frontiers in sustainable food systems, 2018.

    Abstract | Links | BibTeX

    Sarah Azinheiro Alejandro Garrido-Maestu Jorge Barros-Velázquez Marta Prado Joana Carvalho Renato Negrinho

    Novel approach for accurate minute DNA quantification on microvolumetric solutions Journal Article

    Microchemical Journal, 2018.

    Abstract | Links | BibTeX

    Sarah Azinheiro Alejandro Garrido-Maestu Jorge Barros-Velázquez Marta Prado Joana Carvalho Renato Negrinho

    Novel approach for accurate minute DNA quantification on microvolumetric solutions Journal Article

    Microchemical Journal, 2018.

    Abstract | Links | BibTeX

  • 2017

    Joana Carvalho Sara Abalde-Cela Enrique Carbó-Argibay Lorena Dieguez Marek Piotrowski Yury Kolen'ko Marta Prado V Alejandro Garrido-Maestu Sarah Azinheiro

    Combination of microfluidic loop-mediated isothermal amplification with gold nanoparticles for rapid detection of Salmonella spp. in food samples Journal Article

    Frontiers in Microbiology In Press, 2017.

    Abstract | Links | BibTeX

    Joana Carvalho Sara Abalde-Cela Enrique Carbó-Argibay Lorena Dieguez Marek Piotrowski Yury Kolen'ko Marta Prado V Alejandro Garrido-Maestu Sarah Azinheiro

    Combination of microfluidic loop-mediated isothermal amplification with gold nanoparticles for rapid detection of Salmonella spp. in food samples Journal Article

    Frontiers in Microbiology In Press, 2017.

    Abstract | Links | BibTeX

    Azinheiro Carvalho Prado P ; S ; J ; M Garrido-Maestu A.; Fuciños

    Systematic loop-mediated isothermal amplification assays for rapid detection and characterization of Salmonella spp., Enteritidis and Typhimurium in food samples Journal Article

    Food Control, 80 , pp. Pages 297-306, 2017.

    Abstract | Links | BibTeX

    Azinheiro Carvalho Prado P ; S ; J ; M Garrido-Maestu A.; Fuciños

    Systematic loop-mediated isothermal amplification assays for rapid detection and characterization of Salmonella spp., Enteritidis and Typhimurium in food samples Journal Article

    Food Control, 80 , pp. Pages 297-306, 2017.

    Abstract | Links | BibTeX

    Prado Wenger Y ; M ; J Vial S.; Berrahal

    Single-Step DNA Detection Assay Monitoring Dual-Color Light Scattering from Individual Metal Nanoparticle Aggregates Journal Article

    ACS Sensors, 2 (2) , pp. pp 251–256, 2017.

    Abstract | Links | BibTeX

    Prado Wenger Y ; M ; J Vial S.; Berrahal

    Single-Step DNA Detection Assay Monitoring Dual-Color Light Scattering from Individual Metal Nanoparticle Aggregates Journal Article

    ACS Sensors, 2 (2) , pp. pp 251–256, 2017.

    Abstract | Links | BibTeX

  • 2016

    Fernandez-Arguelles Diéguez Fuciños Vial Oliveira Reis Boehme B ; M T ; L ; P ; S ; J M ; R L ; K Prado M.; Espiña

    Detection of Foodborne Pathogens Using Nanoparticles. Advantages and Trends Book Chapter

    Antimicrobial Food Packaging, pp. pp.183-202, Jorge Barros-Velazquez, 1st Edition, 2016, ISBN: 978-0-12-800723-5.

    Abstract | Links | BibTeX

    Fernandez-Arguelles Diéguez Fuciños Vial Oliveira Reis Boehme B ; M T ; L ; P ; S ; J M ; R L ; K Prado M.; Espiña

    Detection of Foodborne Pathogens Using Nanoparticles. Advantages and Trends Book Chapter

    Antimicrobial Food Packaging, pp. pp.183-202, Jorge Barros-Velazquez, 1st Edition, 2016, ISBN: 978-0-12-800723-5.

    Abstract | Links | BibTeX

    Vial Rivas Calo-Mata Barros-Velázquez I ; S ; J ; P ; J Prado M.; Ortea

    Advanced DNA- and Protein-based Methods for the Detection and Investigation of Food Allergens Journal Article

    Critical Reviews in Food Science and Nutrition, 56 (Issue 15), pp. 2511-2542, 2016.

    Abstract | Links | BibTeX

    Vial Rivas Calo-Mata Barros-Velázquez I ; S ; J ; P ; J Prado M.; Ortea

    Advanced DNA- and Protein-based Methods for the Detection and Investigation of Food Allergens Journal Article

    Critical Reviews in Food Science and Nutrition, 56 (Issue 15), pp. 2511-2542, 2016.

    Abstract | Links | BibTeX

GROUP LEADER

Marta-Prado-2_w

THE TEAM

Alejandro Garrido
Staff Researcher

Foteini Roumani
Research Fellow

Agnes Purwidyantri
Research Fellow

Sarah Azinheiro
PhD Student

FORMER GROUP MEMBERS

Carlos Carpena
visitor from Ocupharm diagnostics S.L.

Joana Carvalho
Laboratory Assistant

Joana Guerreiro
Research Fellow

Jon Ashley
Research Fellow

Karola Böhme (Research Fellow)

Gema Puertas

Master student ( February – September 2017)

University de Santiago de Compostela

Danna Sepulveda Sampavo

Visitor (June 2017)

Sofia Granja Martins

Summer Student (July – September 2017)

María Leyva (Scientific Visitor)

Anna Toldrà Filella
Visitor from IRTA

Cristina Pastrana
visitor from Ocupharm diagnostics S.L.

Shambhavi Yadav
Scientific Associate

Monisha Elumalai
Research Fellow

Andrey Ipatov
Research Fellow

NEWS & EVENTS

New funded projects!

  • PORT GRAPHE – Control of Port and Douro Wines authenticity using graphene DNA sensors project
    (Project Time Frame: June 2018 to June 2021)
  • Nano BioSensor – Development of nanosensors to evaluate the microbiological quality of fruit-based products
    (Project Time Frame: July 2018 to June 2021)

Reliable Nano & Micro-solutions for Food Safety & Quality Analysis

The main objective of the Food & Quality Research Group is the development of analytical approaches based on the combination of molecular biology (mainly DNA based methodology) and nano and microfabrication technology in order to provide the food industry and control laboratories with reliable analytical tools.

Following this objective, the methodology is based on working on very specific analytical needs and on using a modular approach for each of the steps of the analytical process. This approach, help us to evaluate and to choose the best method in each case, to have a sound integrated final product, and at the same time a wide-range of intermediate products that can be used by themselves to solve specific analytical challenges.

Figure 1 summarizes the overall approach and research lines. Our topics of interest involve the detection of foodborne pathogens, the detection of allergenic ingredients in food products and food authenticity.

RESEARCH LINES

  • Sample preparation:

Sample preparation is the series of steps required to transform a sample to a form suitable for analysis, the reliability of the conclusions drawn from food analysis greatly depends upon on this step. We work on: (i) the development of pre-treatment steps in order to overcome some of the limitations associated with food analysis and (ii) on the development of tailored, miniaturized, automatized and faster sample preparation techniques. Microscale solid phase extraction (µSPE) is used for on-chip DNA extraction and purification, being possible to put in contact a higher volume of initial binding material with the solid phase and recover the DNA in a lower volume during the elution phase. This feature allows to concentrate the DNA when minute amounts are present in the sample (e.g. olive oil, wine), for complex matrixes such as processed foodstuff and for environmental samples (e.g. water samples).

  • Alternative DNA amplification methods:

Food & Quality Safety Research Group is working on new amplification techniques, in their combination with NPs and on the evaluation of DNA based analytical methods for food analysis. We work on isothermal amplification techniques, such as Loop-Mediated Isothermal Amplification (LAMP), and Recombinase Polymerase Amplification (RPA), specially interesting for miniaturization purposes. Other alternative techniques currently being used include Ligation Chain Reaction (LCR) which allows to distinguish very closely related organisms and high similar DNA sequences.

  • Nanoparticle-assisted DNA analysis:

The use of nanomaterials for DNA analysis has the potential of providing increased sensitivity, multiplexing capabilities, and reduced costs. Exploiting the features of nanoparticles (NPs) is considered to be a good alternative to foster the potential of diagnostics and analytical method development. NPs, such as gold NPs (AuNPs) and gold nanorods (AuNRs) are being used for DNA detection taking advantage of their optical properties.

DEVELOPMENT OF A SYSTEM OF EARLY DETECTION OF THE ZEBRA MUSSEL THROUGH ANALYSIS OF ENVIRONMENTAL DNA

Cooperation Agreement with Confederación hidrográfica del Guadalquivir (CHG) Spain.

The objective of this cooperation agreement is the development of a micro Total Analysis Systems (µTAS) and optimized protocol for the detection of zebra mussel, an invasive species affecting numerous river basins worldwide, through environmental DNA (eDNA).

NANOEATERS: Valorization and transfer of NANOtechnologies to EArly adopTERS of the Euroregion Galicia-Norte Portugal-Use Case 2: Olive Oil Characterization

The FQ&S research group participates on the NANOEATERS project, on the Use Case 2: Olive Oil characterization on collaboration with the University of Vigo (Spain).
The main objective of use case 2 is the characterization of Extra virgin olive oil produced in Galicia, and the development of analytical approaches that would allow the differentiation of such olive oil from the one produced elsewhere.

N2020- NBFS-FOODSAFE

Innovative sample preparation and detection methods for foodborne pathogens, allergenic ingredients and food authenticity assessment are being developed. In parallel, new smart packaging and delivery systems are being designed and validated.

PORTGRAPHE

Control of Port and Douro Wines authenticity using graphene DNA sensors project

Time Frame: June 2018 to June 2021

The main objective of the project is the development, test and in-house validation of a miniaturized DNA sensing device for varietal discrimination of grapes, wines, musts, and grape juice in order to ensure the authenticity of wine from Port and Douro DOP. With this objective the participating teams will combine their expertise for the development of a miniaturized analytical device composed of 3 modules namely: a DNA extraction and purification module, an isothermal DNA amplification module, and a DNA Biosensors based on field-effect transistors (FETs) made using single layer graphene (SLG) for varietal discrimination.

NanoBioSensor

Development of nanosensors to evaluate the microbiological quality of fruit-based products

Time Frame: July 2018 to June 2021

A micro-total analysis system will be developed by the participating team to enable faster and better control of selected fruit-based products, reducing the analysis time from 7 days to few hours with greater sensitivity than conventional methods. This device will be designed and manufactured combining the latest developments in molecular biology, microfluidics and electronics.

SF4SF Smart Factoy for Safe Foods

 

Project funded by CDTI, Ministry of Industry of Spain.

The project aims the integration of emerging technologies for the detection, removal and food hazards management in food processing plants. The role of the group is the development of analytical methods based on DNA detection from gluten producing cereals and Listeria monocytogenes in fish products.

PUBLICATIONS

  • 2021

    Azinheiro, S., Ghimire, D., Carvalho, J., Prado, M., & Garrido-Maestu, A. (2022)

    Next-day detection of viable Listeria monocytogenes by multiplex reverse transcriptase real-time PCR Journal Article

    Food Control, 133 (PA) , 2021.

    Abstract | Links | BibTeX

    Azinheiro, S., Roumani, F., Carvalho, J., Prado, M., & Garrido-Maestu, A.

    Suitability of the MinION long read sequencer for semi-targeted detection of foodborne pathogens Journal Article

    Analytica Chimica Acta, 1184 , 2021.

    Abstract | Links | BibTeX

    Guerreiro, J. R. L., Ipatov, A., Carvalho, J., Toldrà, A., & Prado, M.

    Amplified plasmonic and microfluidic setup for DNA monitoring Journal Article

    Microchimica Acta, (2021), (326), 2021.

    Abstract | Links | BibTeX

    Azinheiro, S., Roumani, F., Rodríguez-Lorenzo, L., Carvalho, J., Prado, M., & Garrido-Maestu, A.

    Combination of Recombinase Polymerase Amplification with SYBR Green I for naked-eye, same-day detection of Escherichia coli O157:H7 in ground meat Journal Article

    Food Control (2022), 132 , 2021.

    Abstract | Links | BibTeX

    Elumalai, M., Ipatov, A., Carvalho, J., Guerreiro, J., Prado, M.

    Dual colorimetric strategy for specific DNA detection by nicking endonuclease-assisted gold nanoparticle signal amplification Journal Article

    Anal Bioanal Chem, 2021.

    Abstract | Links | BibTeX

    Carvalho, J.; Yadav, S.; Garrido-Maestu, A.G.; Azinheiro, S.; Trujillo, I.; Barros-Velázquez, J.; Prado, M.

    Evaluation of simple sequence repeats (SSR) and single nucleotide polymorphism (SNP)-based methods in olive varieties from the Northwest of Spain and potential for miniaturization Journal Article

    Food Chemistry: Molecular Sciences, 3 , 2021.

    Abstract | Links | BibTeX

    Maryam Naseri ,Arnab Halder ,Mohsen Mohammadniaei ,Marta Prado ,Jon Ashley ,Yi Sun

    A multivalent aptamer-based electrochemical biosensor for biomarker detection in urinary tract infection Journal Article

    Electrochimica Acta (2021), 389 , 2021.

    Abstract | Links | BibTeX

    Roumani, F.; Azinheiro, S.; Sousa, H.; Sousa, A.; Timóteo, M.; Varandas, T.; Fonseca-silva, D.; Baldaque, I.; Carvalho, J.; Prado, M.; Garrido-Maestu

    Optimization and Clinical Evaluation of a Multi-Target Loop-Mediated Isothermal Amplification Assay for the Detection of SARS-CoV-2 in Nasopharyngeal Samples Journal Article

    Viruses 2021, 2021.

    Abstract | Links | BibTeX

    Carvalho J and Maestu AG and Azinheiro S and Fuciños P and Velázquez J B and Miguel RJ De and Gros V and Prado M

    Faster monitoring of the invasive alien species ( IAS ) Dreissena polymorpha in river basins through isothermal amplification Journal Article

    Scientific Reports, 11 (10175), 2021.

    Abstract | Links | BibTeX

    Ipatov, A and Garrido- Maestu, A and Guerreiro, J R and Purwidyantri, A and Azinheiro, S and Carvalho, J and Romani, F and Elumalai, M and Prado, M

    Application of Omics-based Miniaturized Systems in Food Quality and Safety Book Chapter

    2021, ISBN: 978-1-78801-884-5.

    Abstract | Links | BibTeX

    Carvalho J and Diéguez L and Ipatov A and Guerreiro J R and Garrido-Maestu A and Azinheiro S and Prado M

    Single-use microfluidic device for purification and concentration of environmental DNA from river water Journal Article

    Talanta, 226 , 2021.

    Abstract | Links | BibTeX

    Purwidyantri A and Domingues T and Borme J and Guerreiro J R and Ipatov A and Abreu C M and Martins M and Alpuim P and Prado M

    Influence of the Electrolyte Salt Concentration on DNA Detection with Graphene Transistors Journal Article

    Biosensors, 11 , pp. 24, 2021.

    Abstract | Links | BibTeX

    Roumani F and Azinheiro S and Carvalho J and Prado M and Garrido-maestu A

    Loop-mediated isothermal amplification combined with immunomagnetic separation and propidium monoazide for the specific detection of viable Listeria monocytogenes in milk products , with an internal amplification control Journal Article

    Food Control, 125 , 2021.

    Abstract | Links | BibTeX

    Jon Ashley, Anna-Lisa Schaap-Johansen, Mohsen Mohammadniaei, Maryam Naseri, Paolo Marcatili, Marta Prado, Yi Sun

    Terminal deoxynucleotidyl transferase-mediated formation of protein binding polynucleotides Journal Article

    Nucleic Acids Research, 2021.

    Abstract | Links | BibTeX

  • 2020

    A Garrido-Maestu and S Azinheiro and F Roumani and J Carvalho and M Prado

    Application of Short Pre-enrichment, and Double Chemistry Real-Time PCR, Combining Fluorescent Probes and an Intercalating Dye, for Same-Day Detection and Confirmation of Salmonella spp. and Escherichia coli O157 in Ground Beef and Chicken Samples Journal Article

    Front. Microbiol, 11 , pp. 1-8, 2020.

    Abstract | Links | BibTeX

    L Wu and A Teixeira and A Garrido-Maestu and L Muinelo-Romay and L Lima and LL Santos and M Prado and L Diéguez

    Profiling DNA mutation patterns by SERS fingerprinting for supervised cancer classification Journal Article

    Biosens. Bioelectron, 165 , 2020.

    Abstract | Links | BibTeX

    S Azinheiro and J Carvalho and M Prado and A Garrido-Maestu

    Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces Journal Article

    Foods, 9 , 2020.

    Abstract | Links | BibTeX

    S Azinheiro and J Carvalho and M Prado

    Multiplex Detection of Salmonella spp., E. coli O157 and L. monocytogenes by qPCR Melt Curve Analysis in Spiked Infant Formula Journal Article

    Microorganisms, 8 , 2020.

    Abstract | Links | BibTeX

    AJN Oliveira and J de Wild and K Oliveira and BA Valença and JP Teixeira and JRL Guerreiro and S Abalde-Cela and TS Lopes and RM Ribeiro and JM V Cunha and MA Curado and M Monteiro and A Violas and AG Silva and M Prado and PA Fernandes and B Vermang and PMP Salomé

    Encapsulation of Nanostructures in a Dielectric Matrix Providing Optical Enhancement in Ultrathin Solar Cells Journal Article

    Sol. RRL., 4 , 2020.

    Abstract | Links | BibTeX

    S Azinheiro and K Kant and MA Shahbazi and A Garrido-Maestu and M Prado and L Diéguez

    A smart microfluidic platform for rapid multiplexed detection of foodborne pathogens Journal Article

    Food Control, 114 , 2020.

    Abstract | Links | BibTeX

    Garrido-Maestu A and Azinheiro S and Carvalho J and Espiña B and Prado M

    Evaluation and implementation of commercial antibodies for improved nanoparticle-based immunomagnetic separation and real-time PCR for faster detection of Listeria monocytogenes Journal Article

    Food Sci. Technol., (57), pp. 4143–4151, 2020.

    Abstract | Links | BibTeX

    A Teixeira and JL Paris and F Roumani and L Diéguez and M Prado and B Espiña and S Abalde-Cela and A Garrido-Maestu and L Rodriguez-Lorenzo

    Multifuntional gold nanoparticles for the SERS detection of pathogens combined with a LAMP-in-microdroplets approach Journal Article

    Materials (Basel), 13 , 2020.

    Abstract | Links | BibTeX

    A Garrido-Maestu and S Azinheiro and J Carvalho and P Fuciños and M Prado

    Optimized sample treatment, combined with real-time PCR, for same-day detection of E. coli O157 in ground beef and leafy greens Journal Article

    Food Control, 108 , 2020.

    Abstract | Links | BibTeX

  • 2019

    L Rodriguez-Lorenzo and A Garrido-Maestu and AK Bhunia and B Espiña and M Prado and L Diéguez and S Abalde-Cela

    Gold Nanostars for the Detection of Foodborne Pathogens via Surface-Enhanced Raman Scattering Combined with Microfluidics Journal Article

    ACS Appl. Nano Mater., 2 (10), pp. 6081–6086, 2019.

    Abstract | Links | BibTeX

    Carla Carvalho Joana Carvalho Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Combination of Immunomagnetic separation and real-time Recombinase Polymerase Amplification (IMS-qRPA) for specific detection of Listeria monocytogenes in smoked salmon samples Journal Article

    Food Science, 84 (7), pp. 1881-1887, 2019.

    Abstract | Links | BibTeX

    Carla Carvalho Joana Carvalho Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Combination of Immunomagnetic separation and real-time Recombinase Polymerase Amplification (IMS-qRPA) for specific detection of Listeria monocytogenes in smoked salmon samples Journal Article

    Food Science, 84 (7), pp. 1881-1887, 2019.

    Abstract | Links | BibTeX

    SarahAzinheiro CarlaCarvalho JoanaCarvalho MartaPrado Alejandro Garrido-Maestu PabloFuciños

    Specific detection of viable Salmonella Enteritidis by phage amplification combined with qPCR (PAA-qPCR) in spiked chicken meat samples Journal Article

    Food Control, 99 , pp. 79-83, 2019.

    Abstract | Links | BibTeX

    SarahAzinheiro CarlaCarvalho JoanaCarvalho MartaPrado Alejandro Garrido-Maestu PabloFuciños

    Specific detection of viable Salmonella Enteritidis by phage amplification combined with qPCR (PAA-qPCR) in spiked chicken meat samples Journal Article

    Food Control, 99 , pp. 79-83, 2019.

    Abstract | Links | BibTeX

    Joana Guerreiro Sandra Carvalho Sara Abalde-Cela Marta Prado Lorena Diéguez R L Lei Wu Alejandro Garrido-Maestu

    Amplification-free SERS analysis of DNA mutation in cancer cells with single-base sensitivity Journal Article

    Nanoscale, 11 (16), pp. 7781-7789, 2019.

    Abstract | Links | BibTeX

    Joana Guerreiro Sandra Carvalho Sara Abalde-Cela Marta Prado Lorena Diéguez R L Lei Wu Alejandro Garrido-Maestu

    Amplification-free SERS analysis of DNA mutation in cancer cells with single-base sensitivity Journal Article

    Nanoscale, 11 (16), pp. 7781-7789, 2019.

    Abstract | Links | BibTeX

  • 2018

    Marta Prado Rodríguez Alejandro Garrido-Maestu David Tomás Fornés

    The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens Book Chapter

    Methods in Molecular Biology, 1918 , pp. 35-45, Humana Press, New York, NY, 2018.

    Abstract | Links | BibTeX

    Marta Prado Rodríguez Alejandro Garrido-Maestu David Tomás Fornés

    The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens Book Chapter

    Methods in Molecular Biology, 1918 , pp. 35-45, Humana Press, New York, NY, 2018.

    Abstract | Links | BibTeX

    SarahAzinheiro AlejandroGarrido-Maestu JorgeBarros-Velázquez MartaPrado JoanaCarvalho RenatoNegrinho

    Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy Journal Article

    Elsevier, 21 (21), pp. 424-431, 2018.

    Abstract | Links | BibTeX

    SarahAzinheiro AlejandroGarrido-Maestu JorgeBarros-Velázquez MartaPrado JoanaCarvalho RenatoNegrinho

    Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy Journal Article

    Elsevier, 21 (21), pp. 424-431, 2018.

    Abstract | Links | BibTeX

    JoãoGaspar SarahAzinheiro LorenaDiéguez AlejandroGarrido-Maestu ManuelVázquez JorgeBarros-Velázquez SusanaCardoso MartaPradoa JoanaCarvalho GemaPuertas

    Highly efficient DNA extraction and purification from olive oil on a washable and reusable miniaturized device Journal Article

    Analytica Chimica Acta, 1020 , pp. 30-40, 2018.

    Abstract | Links | BibTeX

    JoãoGaspar SarahAzinheiro LorenaDiéguez AlejandroGarrido-Maestu ManuelVázquez JorgeBarros-Velázquez SusanaCardoso MartaPradoa JoanaCarvalho GemaPuertas

    Highly efficient DNA extraction and purification from olive oil on a washable and reusable miniaturized device Journal Article

    Analytica Chimica Acta, 1020 , pp. 30-40, 2018.

    Abstract | Links | BibTeX

    Sarah Azinheiro Pablo Fuciños Joana Carvalho Marta Prado Alejandro Garrido-Maestu

    Highly sensitive detection of gluten-containing cereals in food samples by real-time Loop-mediated isothermal AMPlification (qLAMP) and real-time polymerase chain reaction (qPCR) Journal Article

    Food Chemistry, Volume 246 , pp. Pages 156–163, 2018.

    Abstract | Links | BibTeX

    Sarah Azinheiro Pablo Fuciños Joana Carvalho Marta Prado Alejandro Garrido-Maestu

    Highly sensitive detection of gluten-containing cereals in food samples by real-time Loop-mediated isothermal AMPlification (qLAMP) and real-time polymerase chain reaction (qPCR) Journal Article

    Food Chemistry, Volume 246 , pp. Pages 156–163, 2018.

    Abstract | Links | BibTeX

    Joana Carvalho Pablo Fuciños Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Development and evaluation of loop-mediated isothermal amplification, and Recombinase Polymerase Amplification methodologies, for the detection of Listeria monocytogenes in ready-to-eat food samples Journal Article

    Food Control, Volume 86 , pp. Pages 27–34, 2018.

    Abstract | Links | BibTeX

    Joana Carvalho Pablo Fuciños Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Development and evaluation of loop-mediated isothermal amplification, and Recombinase Polymerase Amplification methodologies, for the detection of Listeria monocytogenes in ready-to-eat food samples Journal Article

    Food Control, Volume 86 , pp. Pages 27–34, 2018.

    Abstract | Links | BibTeX

    Joana Carvalho Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Rapid and sensitive detection of viable Listeria monocytogenes in food products by a filtration-based protocol and qPCR Journal Article

    Food Microbiology, 73 , pp. 254-263, 2018.

    Abstract | Links | BibTeX

    Joana Carvalho Marta Prado Alejandro Garrido-Maestu Sarah Azinheiro

    Rapid and sensitive detection of viable Listeria monocytogenes in food products by a filtration-based protocol and qPCR Journal Article

    Food Microbiology, 73 , pp. 254-263, 2018.

    Abstract | Links | BibTeX

    Marta Prado Sarah Azinheiro Joana Carvalho and Alejandro Garrido-Maestu.

    Evaluation of different genetic targets for Salmonella enterica serovar Enteriditis and Typhimurium, using Loop-mediated isothermal AMPlification for detection in food samples Journal Article

    Frontiers in sustainable food systems, 2018.

    Abstract | Links | BibTeX

    Marta Prado Sarah Azinheiro Joana Carvalho and Alejandro Garrido-Maestu.

    Evaluation of different genetic targets for Salmonella enterica serovar Enteriditis and Typhimurium, using Loop-mediated isothermal AMPlification for detection in food samples Journal Article

    Frontiers in sustainable food systems, 2018.

    Abstract | Links | BibTeX

    Sarah Azinheiro Alejandro Garrido-Maestu Jorge Barros-Velázquez Marta Prado Joana Carvalho Renato Negrinho

    Novel approach for accurate minute DNA quantification on microvolumetric solutions Journal Article

    Microchemical Journal, 2018.

    Abstract | Links | BibTeX

    Sarah Azinheiro Alejandro Garrido-Maestu Jorge Barros-Velázquez Marta Prado Joana Carvalho Renato Negrinho

    Novel approach for accurate minute DNA quantification on microvolumetric solutions Journal Article

    Microchemical Journal, 2018.

    Abstract | Links | BibTeX

  • 2017

    Joana Carvalho Sara Abalde-Cela Enrique Carbó-Argibay Lorena Dieguez Marek Piotrowski Yury Kolen'ko Marta Prado V Alejandro Garrido-Maestu Sarah Azinheiro

    Combination of microfluidic loop-mediated isothermal amplification with gold nanoparticles for rapid detection of Salmonella spp. in food samples Journal Article

    Frontiers in Microbiology In Press, 2017.

    Abstract | Links | BibTeX

    Joana Carvalho Sara Abalde-Cela Enrique Carbó-Argibay Lorena Dieguez Marek Piotrowski Yury Kolen'ko Marta Prado V Alejandro Garrido-Maestu Sarah Azinheiro

    Combination of microfluidic loop-mediated isothermal amplification with gold nanoparticles for rapid detection of Salmonella spp. in food samples Journal Article

    Frontiers in Microbiology In Press, 2017.

    Abstract | Links | BibTeX

    Azinheiro Carvalho Prado P ; S ; J ; M Garrido-Maestu A.; Fuciños

    Systematic loop-mediated isothermal amplification assays for rapid detection and characterization of Salmonella spp., Enteritidis and Typhimurium in food samples Journal Article

    Food Control, 80 , pp. Pages 297-306, 2017.

    Abstract | Links | BibTeX

    Azinheiro Carvalho Prado P ; S ; J ; M Garrido-Maestu A.; Fuciños

    Systematic loop-mediated isothermal amplification assays for rapid detection and characterization of Salmonella spp., Enteritidis and Typhimurium in food samples Journal Article

    Food Control, 80 , pp. Pages 297-306, 2017.

    Abstract | Links | BibTeX

    Prado Wenger Y ; M ; J Vial S.; Berrahal

    Single-Step DNA Detection Assay Monitoring Dual-Color Light Scattering from Individual Metal Nanoparticle Aggregates Journal Article

    ACS Sensors, 2 (2) , pp. pp 251–256, 2017.

    Abstract | Links | BibTeX

    Prado Wenger Y ; M ; J Vial S.; Berrahal

    Single-Step DNA Detection Assay Monitoring Dual-Color Light Scattering from Individual Metal Nanoparticle Aggregates Journal Article

    ACS Sensors, 2 (2) , pp. pp 251–256, 2017.

    Abstract | Links | BibTeX

  • 2016

    Fernandez-Arguelles Diéguez Fuciños Vial Oliveira Reis Boehme B ; M T ; L ; P ; S ; J M ; R L ; K Prado M.; Espiña

    Detection of Foodborne Pathogens Using Nanoparticles. Advantages and Trends Book Chapter

    Antimicrobial Food Packaging, pp. pp.183-202, Jorge Barros-Velazquez, 1st Edition, 2016, ISBN: 978-0-12-800723-5.

    Abstract | Links | BibTeX

    Fernandez-Arguelles Diéguez Fuciños Vial Oliveira Reis Boehme B ; M T ; L ; P ; S ; J M ; R L ; K Prado M.; Espiña

    Detection of Foodborne Pathogens Using Nanoparticles. Advantages and Trends Book Chapter

    Antimicrobial Food Packaging, pp. pp.183-202, Jorge Barros-Velazquez, 1st Edition, 2016, ISBN: 978-0-12-800723-5.

    Abstract | Links | BibTeX

    Vial Rivas Calo-Mata Barros-Velázquez I ; S ; J ; P ; J Prado M.; Ortea

    Advanced DNA- and Protein-based Methods for the Detection and Investigation of Food Allergens Journal Article

    Critical Reviews in Food Science and Nutrition, 56 (Issue 15), pp. 2511-2542, 2016.

    Abstract | Links | BibTeX

    Vial Rivas Calo-Mata Barros-Velázquez I ; S ; J ; P ; J Prado M.; Ortea

    Advanced DNA- and Protein-based Methods for the Detection and Investigation of Food Allergens Journal Article

    Critical Reviews in Food Science and Nutrition, 56 (Issue 15), pp. 2511-2542, 2016.

    Abstract | Links | BibTeX

GROUP LEADER

THE TEAM

Alejandro Garrido
Staff Researcher

Joana Guerreiro
Research Fellow

Monisha Elumalai
Research Fellow

Andrey Ipatov
Research Fellow

Jon Ashley
Research Fellow

Foteini Roumani
Research Fellow

Sarah Azinheiro
Research Laboratory Assistant

Joana Carvalho
Research Laboratory Assistant

Carlos Carpena
visitor from Ocupharm diagnostics S.L.

Shambhavi Yadav
Scientific Associate

FORMER GROUP MEMBERS

Karola Böhme (Research Fellow)

Gema Puertas

Master student ( February – September 2017)

University de Santiago de Compostela

Danna Sepulveda Sampavo

Visitor (June 2017)

Sofia Granja Martins

Summer Student (July – September 2017)

María Leyva (Scientific Visitor)

Anna Toldrà Filella
Visitor from IRTA

Cristina Pastrana
visitor from Ocupharm diagnostics S.L.

RESEARCH

HIGHLIGHTS

Paper published in Food Microbiology

Rapid and sensitive detection of viable Listeria monocytogenes in food products by a filtration-based protocol and qPCR. Alejandro Garrido-Maestu, , Sarah Azinheiro, Joana Carvalho, Marta Prado. Volume 73, August 2018, Pages 254–263.

Published a Research Article in Front. Sustain. Food Syst.

Evaluation of Different Genetic Targets for Salmonella enterica Serovar Enteriditis and Typhimurium, Using Loop-Mediated Isothermal AMPlification for Detection in Food Samples

SarahAzinheiro,JoanaCarvalho,MartaPrado and AlejandroGarrido-Maestu. 21 February 2018.

Paper published in Microchemical Journal

Novel approach for accurate minute DNA quantification on microvolumetric solutions JoanaCarvalho,RenatoNegrinho,SarahAzinheiro,AlejandroGarrido-Maestu,JorgeBarros-Velázquez.MartaPrado.Volume 138, May 2018, Pages 540-549.

Paper published in Analytica chimica Acta.

Highly efficient DNA extraction and purification from olive oil on a washable and reusable miniaturized device.

JoanaCarvalho, GemaPuertas, JoãoGaspar, SarahAzinheiro, LorenaDiéguez, AlejandroGarrido-Maestu, ManuelVázquez,JorgeBarros-Velázquez,SusanaCardoso,MartaPrado.Volume 1020, 22 August 2018, Pages 30-40

NEWS & EVENTS

Specific detection of viable Salmonella Enteritidis by phage amplification combined with qPCR (PAA-qPCR) in spiked chicken meat samples

Author: Alejandro Garrido-Maestu, PabloFuciños, SarahAzinheiro, CarlaCarvalho, JoanaCarvalho, MartaPrado

Food Control 99 , pp. 79-83, 2019.

Book Chapter in collaboration with David Tomás Fornés, Lead Scientist in the Microbial and Molecular Analytics group at Nestlé Research Center (Lausanne, Switzerland). !!!!!! Foodborne Bacterial Pathogens

The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens In: Bridier A. (eds) Foodborne Bacterial Pathogens. Methods in Molecular Biology, vol 1918. Humana Press, New York, NY

New funded projects!

  • PORT GRAPHE – Control of Port and Douro Wines authenticity using graphene DNA sensors project
    (Project Time Frame: June 2018 to June 2021)
  • Nano BioSensor – Development of nanosensors to evaluate the microbiological quality of fruit-based products
    (Project Time Frame: July 2018 to June 2021)