Diéguez Group
The Diéguez Research Group works at the interface of research, technology development, and the clinic with the overarching goal of translating science to improve of people’s health. The group capitalises on the unparalleled control over fluid dynamics, high-throughput, and reproducibility offered by microfluidics, and on the high sensitivity and specificity of nanobiosensors to develop innovative solutions aiming to further our current understanding of human diseases leading to better treatment and earlier diagnostics.
Research lines:
- Biomarker enrichment and isolation from liquid biopsies toward minimally invasive disease monitoring and diagnosis
- Ultrasensitive detection using nanobiosensors toward earlier and accurate disease diagnosis
- Advanced 3D models based on multicellular spheroids, organoids, and organ-on-chip toward disease modelling and precision medicine
- Development of advanced Medical Instrumentation based on optical technologies
Projects
Publications
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Advances in Microfluidics for the Implementation of Liquid Biopsy in Clinical Routine
Microfluidics and Biosensors in Cancer Research pp 553–590, 2022A Phage Receptor-Binding Protein as a Promising Tool for the Detection of Escherichia coli in Human Specimens
FRONTIERS IN MICROBIOLOGY, 2022Burden of bacterial bloodstream infections and recent advances for diagnosis
PATHOGENS AND DISEASE, 2022Establishing an expert consensus on key indicators of quality of life among breast cancer survivors: a modified Delphi study
Journal of Clinical Medicine 2022, 11 (7), 2041, 2022Potential of bacteriophage proteins as recognition molecules for pathogen detection.
Critical Reviews in Biotechnology, 2022 -
Use of some cost-effective technologies for a routine clinical pathology laboratory
LAB ON A CHIP, 2021Correlative atomic force microscopy
Published May 2021 • Copyright © IOP Publishing Ltd 2021 Pages III.1.b-1 to III.1.b-13, 2021Combining magnetic and optical approaches for the multiplex detection of bacteria in blood
World Microbe Forum, 2021Front Cover of the article Fluorescence cross-correlation spectroscopy as a valuable tool to characterize cationic liposome-DNA nanoparticle assembly
Journal of Biophotonics - First published: 04 January 2021, 2021Inside back cover - Showcasing a collaborative review work between the laboratory of Dr Pieter De Beule with Dr Andreas Stylianou, Dr Liisa Hirvonen and Dr Humberto Sánchez. How did correlative atomic force microscopy and super- resolution microscopy evolve in the quest for unravelling enigmas in biology?
Nanoscale, 2021,13, 2729-2729 , 2021 -
Fluorescencecross-correlationspectroscopy as a valuable tool to characterize cationicliposome-DNAnanoparticle assembly
JOURNAL OF BIOPHOTONICS, 2020A smart microfluidic platform for rapid multiplexed detection of foodborne pathogens
FOOD CONTROL, 2020In Vitro Evaluation of Lipopolyplexes for Gene Transfection: Comparing 2D, 3D and Microdroplet-Enabled Cell Culture
MOLECULES, 2020Profiling DNA mutation patterns by SERS fingerprinting for supervised cancer classification
BIOSENSORS & BIOELECTRONICS, 2020Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
SCIENTIFIC REPORTS, 2020 -
Functionalization of single-layer graphene for immunoassays
APPLIED SURFACE SCIENCE, 2019Microfluidics-Driven Fabrication of a Low Cost and Ultrasensitive SERS-Based Paper Biosensor
APPLIED SCIENCES-BASEL, 2019The Significance of Circulating Tumour Cells in the Clinic
ACTA CYTOLOGICA, 2019Attomolar Label-Free Detection of DNA Hybridization with Electrolyte-Gated Graphene Field-Effect Transistors
ACS SENSORS, 2019 -
High frequency FM MEMS accelerometer Using piezoresistive resonators
Proc. 31th Eur. Conf. Solid-State Transducers (Eurosensors XXXII), Graz, Austria, Sept. 9-12, 2018, 1048 (4 pp), 2018Enhanced magnetic microcytometer with 3D flow focusing for cell enumeration
LAB ON A CHIP, 2018Wafer scale fabrication of graphene microelectrode arrays for the detection of DNA hybridization
MICROELECTRONIC ENGINEERING, 2018 -
Challenges and trends in magnetic sensor integration with microfluidics for biomedical applications
JOURNAL OF PHYSICS D-APPLIED PHYSICS, 2017Spintronic biochips: from the laboratory to pre-clinical applications
Nanomagnetism: Applications and Perspectives, First Edition. Edited by Claude Fermon, 2017Adapting Bobbert-Vlieger model to spectroscopic ellipsometry of gold nanoparticles with bio-organic shells
BIOMEDICAL OPTICS EXPRESS, 2017 -
Lipoplexes for Gene Delivery Characterized by Fluorescence Correlation Spectroscopy
BIOPHYSICAL JOURNAL, 2016Simultaneous Advanced Microscopies for Live Cell Signaling Dynamics Investigations
BIOPHYSICAL JOURNAL, 2016Microscopic thin film optical anisotropy imaging at the solid-liquid interface
REVIEW OF SCIENTIFIC INSTRUMENTS, 2016 -
Simultaneous differential spinning disk fluorescence optical sectioning microscopy and nanomechanical mapping atomic force microscopy
REVIEW OF SCIENTIFIC INSTRUMENTS, 2015Efficient microfluidic negative enrichment of circulating tumor cells in blood using roughened PDMS
Analyst 140, 3565-3572, 2015, 2015Design and fabrication of SiO2/TiO2 and MgO/TiO2 based high selective optical filters for diffuse reflectance and fluorescence signals extraction
BIOMEDICAL OPTICS EXPRESS, 2015 -
Robust and Flexible Fabrication of Chemical Micropatterns for Tumor Spheroid Preparation
ACS Appl. Mater. Interfaces 6, 13, 10162-10171, 2014 , 2014Optical Scattering of Liposomes Suspended above a Surface
BIOPHYSICAL JOURNAL, 2014Surface scattering of core-shell particles with anisotropic shell
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2014Towards Far-Field Microscopic Imaging of Supported Lipid Bilayer Optical Anisotropy
BIOPHYSICAL JOURNAL, 2014